Measurement of cytokines secreted into the culture medium by ELISA. Measurement of IFN-γ, IL-2, TNF-α, IL-12, and IL-1β secreted in the culture medium from different groups of cells at day 1, 3 and 9. Representative data from three individual experiments of a single donor are shown. Unpaired Student’s t-test was performed. NS: not significant, *p < 0.001, **p < 0.01.