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Table 1 Real-time PCR primers and probes[1618]

From: Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis

Oligonuclotide Sequence Final conc. (nM)
ctrA forward 5′-TGTGTTCCGCTATACGCCATT-3′ 300
ctrA reverse 5′-GCCATATTCACACGATATACC-3′ 900
ctrA probe 5′-FAM-AACCTTGAGCAA″T″CCATTTATCCTGACGTTCT-3′-SpC6* 100
bexA forward 5′-TGCGGTAGTGTTAGAAAATGGTATTATG-3′ 600
bexA reverse 5′-GGACAAACATCACAAGCGGTTA-3′ 600
bexA probe 5′-FAM-ACAAAGCGTATCAA″T″ACTACAACGAGACGCAAAAA-3′-SpC6* 100
lytA forward 5′-ACGCAATCTAGCAGATGAAGCA-3′ 200
lytA reverse 5′-TCGTGCGTTTTAATTCCAGCT 200
lytA probe 5′-FAM-TGCCGAAAACGCTTGATACAGGGAG-3′-BHQ1 200
  1. * The quencher BHQ-1 is attached to the internal thymine residue “T”.