Toxoplasma gondii cathepsin proteases are undeveloped prominent vaccine antigens against toxoplasmosis

BMC Infectious Diseases

Table 3 IC ₅₀ values for TgCPB and TgCPL peptide binding to MHC class II molecules obtained using the immune epitope database

MHC II Allele¹	Start-Stop²	Sequence³	Percentile Rank⁴
TgCPB
HLA-DRB1*01:01	10–24	LGTPLPFAALAAILL	0.01
HLA-DRB1*01:01	71–85	ESLFDSIVAEQVVAT	6.21
HLA-DRB1*01:01	228–242	LRFRYLSLKDAKKLM	4.99
HLA-DRB1*01:01	320–334	SQGKRLMPLSAQHTT	2.74
HLA-DRB1*01:01	470–484	SGVYKHVSGLPVGGH	1.58
H2-IAb	8–22	RVLGTPLPFAALAAI	3.83
H2-IAb	59–73	DVLNAFVSPESVESL	3.51
H2-IAb	187–201	VFWESRPASSNAAVA	0.91
H2-IAb	300–314	CGSCWAFASTEAFND	4.46
H2-IAb	549–563	LPGQRAAGARAGAHA	3.19
H2-IAd	15–29	PFAALAAILLLGCMY	1.88
H2-IAd	191–205	SRPASSNAAVALIKK	4.34
H2-IAd	227–241	SLRFRYLSLKDAKKL	1.21
H2-IAd	320–334	SQGKRLMPLSAQHTT	3.19
H2-IAd	428–442	QDTHKATSAYSLRSR	2.52
H2-IEd	182–196	ETGEDVFWESRPASS	17.89
H2-IEd	226–240	VSLRFRYLSLKDAKK	13.43
H2-IEd	351–365	QPGMAWRWFERKGVV	4.77
H2-IEd	399–413	DATLVPRKTPKCRKD	16.82
H2-IEd	432–446	KATSAYSLRSRDDVK	9.89
TgCPL
HLA-DRB1*01:01	60–74	RAWIALVAAAVSLLV	0.04
HLA-DRB1*01:01	46–60	YFWKKFLRQRNFTAR	6.00
HLA-DRB1*01:01	117–131	FSSFQAMYAKSYATE	2.74
HLA-DRB1*01:01	136–150	RRYAIFKNNLVYIHT	3.24
HLA-DRB1*01:01	178–192	LGFKKSRNLKSHHLG	5.20
H2-IAb	27–41	RRGVRAGRPSPPFVV	1.49
H2-IAb	59–73	ARAWIALVAAAVSLL	3.37
H2-IAb	85–99	EDDRAVFPPSPVEDH	3.42
H2-IAb	116–130	AFSSFQAMYAKSYAT	5.68
H2-IAb	226–240	CGSCWAFSTTGALEG	2.49
H2-IAd	22–36	GELHQRRGVRAGRPS	6.17
H2-IAd	57–71	FTARAWIALVAAAVS	0.14
H2-IAd	117–131	FSSFQAMYAKSYATE	2.63
H2-IAd	318–332	DVPRRSEAAMKAALA	0.01
H2-IAd	395–409	RDGYMYMAMHKGEEG	1.82
H2-IEd	42–56	TTRTYFWKKFLRQRN	7.05
H2-IEd	174–188	RRKYLGFKKSRNLKS	13.03
H2-IEd	202–216	PSELPAGVDWRSRGC	28.28
H2-IEd	309–323	KVVKILGFKDVPRRS	21.70
H2-IEd	376–390	KESKKDFWIMKNSWG	24.70

¹ H2-IAb, H2-IAd and H2-IEd alleles are mouse MHC class II molecules; the HLA-DRB1*01:01 allele is a human MHC class II molecule.
² We chose 15 amino acids for analysis each time.
³ TgCPB or TgCPL sequences.
⁴ Low percentile = high binding.

ISSN: 1471-2334