In situ molecular identification of the Influenza A (H1N1) 2009 Neuraminidase in patients with severe and fatal infections during a pandemic in Mexico City

Table 2 Clinical data of influenza illness patients

Patient	Fever	Cough	Headache	Sore throat	Rinorrhea	Myalgia	Chills	Nasal Congestion	Conjunctivitis	A (H1N1) Conventional Methods (RT-PCR standard protocols)	A (H1N1) in situ RT-PCR
P01	+	+	+	+	-	+	+	+	+	+	+
P02	+	+	+	+	+	-	+	-	-	+	+
P03	+	+	-	-	+	+	-	-	-	+	+
P04	+	+	+	+	+	+	+	-	-	+	+
P05	+	-	+	-	-	-	-	+	+	+	+
P06	+	+	+	-	+	+	-	-	-	+	+
P07	+	+	+	+	+	-	+	-	-	+	+
P08	+	+	-	+	-	+	-	+	+	+	+
Positive Control 1 [A (H1N1) laboratory strain]	ND	ND	ND	ND	ND	ND	ND	ND	ND	+	+
Positive Control 2 [A (H1N1) positive patient]	+	+	+	-	-	+	+	+	+	+	ND
Negative Control 1 (Influenza-negative Patient 1)	-	-	-	-	-	-	-	-	-	-	-
Negative Control 2 (Influenza-negative Patient 2)	-	-	-	-	-	-	-	-	-	-	-
Negative Control (HeLa cervical cell line)	ND	ND	ND	ND	ND	ND	ND	ND	ND	-	-

ISSN: 1471-2334