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Table 3 ELISA results for melioidosis confirmed and control sera

From: Multiple-antigen ELISA for melioidosis - a novel approach to the improved serodiagnosis of melioidosis

Recombinant proteins Cut-off Sensitivity (%) (no. positive/total no.*) Specificity (%) (no. positive/total no. †)
   Culture confirmed IFAT confirmed Combined  
   (n = 42) (n = 26) (n = 68)  
TssD-5 (type VI secretion system HCP protein) 0.1094 62 (26/42) 85 (22/26) 71 (48/68) 96 (4/90)
Omp3 (outer membrane protein A) 0.1387 50 (21/42) 73 (19/26) 59 (40/68) 90 (9/90)
smBpF4 (serine protease MprA) 0.1269 38 (16/42) 46 (12/26) 41 (28/68) 89 (10/90)
Omp85 (outer membrane protein) 0.2030 19 (8/42) 19 (5/26) 19 (13/68) 96 (4/90)
Cocktails of 4 recombinant proteins§ 0.1936 64 (27/42) 65 (17/26) 65 (44/68) 99 (1/82**)
Multiple antigens combination (all 4 recombinant proteins‡)     88.2 (60/68) 96 (4/90)
  1. * melioidosis confirmed sera by ELISA against B. pseudomallei lysate.
  2. † non-melioidosis sera [healthy control (n = 61) and disease control (n = 29)].
  3. ** non-melioidosis sera [healthy control (n = 59) and disease control (n = 23)].
  4. § Cocktail of 4 recombinant proteins used to coat a single well.
  5. ‡ The single antigen ELISA data was re-evaluated and different criteria were used to calculate sensitivity and specificity. The serum tested was determined to be melioidosis positive according to the following criteria: (1) any 2 or more antigens specifically react with serum with the absorbance greater than the cut-off value calculated from the mean absorbance (A405nm) plus 2 standard deviations of healthy controls or (2) any antigen that specifically reacts with serum when the cut-off value was calculated from the mean absorbance (A405nm) plus 3 standard deviations of healthy controls.
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