Reactivity of human sera with 4 recombinant
proteins. Purified recombinant (A) TssD-5, (B) Omp3, (C) smBpF4 and (D) Omp85 were screened against melioidosis patients’ (n = 68, solid circle), healthy control (n = 61, solid square) and disease control (n = 29, solid triangle) sera. Each symbol represents the average of triplicate readings of one serum sample. Shown is a representative of 2 independent experiments. Cut-off values indicated by dashed line. Significance determined using the Mann Whitney test (***P-value < 0.0001; **P-value < 0.001; *P-value < 0.01).