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Table 4 ITS-1 sequencing for validation of Leishmania species identity in kDNA / RT-PCR positive samples

From: Evaluation of PCR procedures for detecting and quantifying Leishmania donovani DNA in large numbers of dried human blood samples from a visceral leishmaniasis focus in Northern Ethiopia

Number

Parasite /ml

Identified species

Notes

1

56

L.major

Sequencing of cloned ITS1 amplicon

2

87

L. donovani

 

3

65

L. donovani

 

4

36

L. donovani

 

5

23

L. donovani

 

6

1993

L. donovani

 

7

69

L. donovani

Direct sequencing

8

552

L.major

 

9

577

L. donovani

 

10

584

L. donovani

 

11

643

L. donovani

 

12

1022

L. donovani

 

13

1180

L. donovani

 

14

1314

L. donovani

 

15

1397

L. donovani

 

16

8923

L. donovani

 

17

11735

L. donovani

 

18

11753

L. donovani

 

19

11973

L. donovani

 

20

11988

L. donovani

 

21

30770

L. donovani

 

22

47851

L. donovani

 
  1. Twenty two samples with differing parasite loads were selected for amplification and sequencing. 20/22 samples proved to be L. donovani while two were L. major infections.