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Table 2 Reexamination of qRT-kDNA PCR results from the cohort study

From: Evaluation of PCR procedures for detecting and quantifying Leishmania donovani DNA in large numbers of dried human blood samples from a visceral leishmaniasis focus in Northern Ethiopia

1

2

3

4

5

Category parasites /ml

1st cohort study kDNA RT-PCR

Retested by kDNA RT-PCR

kDNA RT-PCR + (current study)

Level of uniformity

0

4,076

107

9

91.6%

1-10

468

108

64

59.3%

11-100

93

48

41

85.4%

101-1000

96

24

23

95.8%

Above 1000

23

19

19

100%

  1. Some of the qRT-kDNA PCR results from the cohort study (Column 2) were reexamined using the same t2:2 protocols (Column 3) the samples that were positive upon re-examination depicted in Column 4. Levels of uniformity (Column 5) indicate the percentage of samples that gave the same result in both tests. Negative and high positive samples were highly consistent. Very low positive samples (1–10 parasites/ml) less so.
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BMC Infectious Diseases

ISSN: 1471-2334

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