Evaluation of PCR procedures for detecting and quantifying Leishmania donovani DNA in large numbers of dried human blood samples from a visceral leishmaniasis focus in Northern Ethiopia

Table 1 PCR systems and primer sets used for the real time kDNA and ITS1 PCR amplification

PCR system	Primers	DNA sequence	Amplicon size (bp)	Ref.
kDNA minicircle	JW11	CCTATTTTACACCAACCCCCAGT	120	[12]
	JW12	GGGTAGGGGCGTTCTGCGAAA
ITS1 PCR	L5.8S	TGATACCACTTATCGCACTT	320	[15]
	LITSR	CTGGATCATTT-TCCGATG

ISSN: 1471-2334