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Table 2 Brucella DNA amplification using B4/B5 and species-specific multiplex PCR

From: Two-stage PCR assay for detection of human brucellosis in endemic areas

(AFI) Samples locality Brucellagenus B4/B5 conventional PCR Brucellaspecies-specific multiplex
(Western region) (number) -ve amplification +ve amplification Double product Single product Single product
Non- Brucellapatients Brucellapatients B. abortus & B. melitensis B. abortus B. melitensis
(Northern region) (61) 11 50 41 (82%) 5 (10%) 4 (8%)
(Central region) (40) 40 ------- Not applicable