Agarose gel electrophoresis of species-specific multiplex assay products. Lane #1 B. melitensis only (252 bp). Lanes 5 and 6, B. abortus only (113 bp); lanes 2, 3, 4, 7, 8, and 9, both of B. abortus and B. melitensis (113 bp and 252 bp respectively). Lane (+ve) and (−ve) contain positive and negative PCR controls. Lane (M) contains a 100-bp ladder (HyperLadder, Bioline, Inc., MA, USA).