Figure 2

Differential M . tuberculosis induced SOCS1 and SOCS3 expression with varying severity of tuberculosis. PBMCs from endemic controls (EC, n = 15) and moderately advanced PTB (PTB-mod, n = 20), far advanced PTB (PTB-adv, n = 13), less severe ETB (L-ETB, n = 26) and severe ETB (D-ETB, n = 7) patients were stimulated with M. tuberculosis H37Rv at MOI-2.5 for 18 hours. Total cellular RNA was harvested and subjected to RT-PCR. mRNA expression levels of IFN-γ, SOCS1, SOCS3 and FoxP3 genes from individual subjects are depicted as scatter plots for each group with median values indicated by a horizontal line. Data shows (A) IFN-γ, (B) SOCS1, (C) SOCS3 and (D) FoxP3 mRNA expression in PBMCs. ‘#’ denotes significant differences (p ≤ 0.05) as compared with EC, ‘*’ denotes significant differences (p ≤ 0.05) between PTB-adv, L-ETB and D-ETB group using Mann–Whitney U non-parametric test.