Figure 1

Differential SOCS1 and FoxP3 gene expression in PTB and ETB. PBMCs from endemic controls (EC, n = 15) and TB patients classified into pulmonary TB (PTB, n = 33) and extra-pulmonary TB (ETB, n = 33) patients were stimulated with M. tuberculosis H37Rv at MOI-2.5 for 18 hours. Total cellular RNA was harvested and subjected to RT-PCR. mRNA expression levels of IFN-γ, SOCS1, SOCS3 and FoxP3 genes from individual subjects were determined. Box and whiskers plots indicate data between 10^th- 90^th percentiles with median values of each group indicated by a horizontal line. Data shows (A) IFN-γ, (B) SOCS1, (C) SOCS3 and (D) FoxP3 mRNA expression in PBMCs. ‘*’ denotes significant differences (p ≤ 0.05) as compared with EC using Mann–Whitney U non-parametric test.