Table 1 Summary of M.tb test targets

Rv0447c

NP854118 (Pool 1)

Probable cyclopropane fatty acyl phospholipid synthase.

427

Methyl transferase activity. Cyclopropane fatty acyl phospholipid synthase activity. Lipid biosynthetic process [17, 18]

Rv2940c

YP_976584 (Pool 2)

Mycocerosic acid synthase

2111

Lipid biosynthetic process. Oxido-reduction and transferase activity, Cofactor binding. Location at the cell wall. [17, 19–23]

Rv3347c

YP_177963 (Pool 3)

PPE family protein

3157

Function unknown. Gly-Ala-Asn rich protein, interacts with the host system by inhibition of antigen processing. [18, 23–26]

Rv2453c

CAA16030 (Pool 4)

Probable molybdopterin-guanine dinucleotide biosynthesis Protein A

201

Molybdenum cofactor biosynthesis. Molecular function as GTP binding. Located at the cytoplasm membrane. [18, 23, 24, 27]

Rv1886c

CAB10044 (Pool 5)

Antigen 85B

325

Fibronectin binding protein. Acyltransferase activity. Secreted protein, also located at cell wall, plasma membrane. [18, 28–31]

Rv1690

CAB10947 (Pool 6)

Probable lipoprotein

127

Putative uncharacterized protein. Protein binding, cellular component plasma membrane.[18, 24, 32]

Rv3019c

CAA16104 (Pool 7)

ESAT-6 like protein

96

Belongs to ESAT-6 (esx) family, Protein-protein interaction [18, 24]

Rv2957

CAB05419 (Pool 8)

PGL/p-HBAD biosynthesis glycosyltrans-ferase MT3031

256

Glycosyl transferase activity, transferring hexosyl groups. Glycolipid biosynthetic function. Identified as a drug target. [18, 24, 30, 33, 34]

Rv1085c

CAA17201 (Pool 9)

UPF0073 membrane protein

242

Belongs to the UPF0073 (HIy-III) family. Cytolysis. Sub cellular location in cell membrane [18, 24]

Rv0066c

CAA16247 (Pool 10)

Isocitrate dehydrogenase, NADP-dependent- icd2.

745

Oxidoreductase function. NAD or NADH binding. Isocitrate dehidrogenase (NADP+) activity. Magnesium ion binding. Protein homodimerization. [18, 22–24, 35, 36]

Rv2958c

CAB05418 (Pool 11)

PGL/p-HBAD biosynthesis glycosyltrans-ferase

428

Glycolipid biosynthetic process, pathogenesis, Glycosyl transferase activity. Immune evasion or - tolerance [18, 22, 24, 33, 37]

Rv2962c

CAB05415 (Pool 12)

PGL/p-HBAD biosynthesis rhamnosyl-transferase

449

Glycolipid biosynthetic process, pathogenesis. Glycosyl transferase activity. Evasion or tolerance concerning to the host immune response. [18, 22, 24, 30, 33, 37]

Rv3804c/1886

CAA17868/CAB10044

Ag85A/Ag85B Fibronecting binding protein peptide pool

338/325

Belongs to the Ag85 family, contains Esterase D. Possesses mycolyl transferase activity. [28, 29, 38–41]

Proteins

Rv3804c

CAA17868

Ag85A. Secreted antigen 85A. Mycolyl transferase 85A. fbpA. Ag85 complex

338

Responsible for high affinity of mycobacteria to fibronectin. Possesses mycolyl transferase for biogenesis of trehalose dimycolate. [22, 23, 29, 30, 38–40, 42–44]

Rv1886c

CAB10044

Ag85B. Secreted antigen 85B. Mycolyl transferase 85B. fbpB. Ag85 complex

325

Fibronectin binding protein. Acyltransferase activity. Response to antibiotic. Secreted protein also located at cell wall, extracellular region. [28–30, 36, 38, 40, 41, 45]

Rv3875

CAA56099

ESAT-6. 6 KDa early secretory antigenic target. esxA

95

Function unknown. Elicit high level of IFNgamma during the first phase of protective immune response. Secreted protein, cell wall and cytoplasm. [46–54]

Rv3874

CAA17966

CFP-10. 10 KDa culture filtrate antigen

100

ESAT-6 like protein esxB. Forms a tight 1:1 complex with EsxA. Protein binding. Host cell surface binding. Pathogenesis. Protein secretion. [22, 47–49, 51, 53, 54]

Rv0754

CAE55319

PE_PGR11. PE-PGRS family protein

584

Member of M.tb PE family. PGRS subfamily gly-rich proteins. Unknown function. Protein existence predicted. [18, 21]

Rv0978c

CAE55343

PE_PGR17. PE-PGRS family protein

331

Member of M.tb PE family. PGRS subfamily gly-rich proteins. Unknown function. Protein existence predicted. [18, 55, 56]

Rv1917c

CAE55440

PPE34. PPE family protein

1459

Member of M.tb PPE family. Glycin rich proteins. Unknown function. [18, 57]

Rv0288

CAA17363

TB10.4. Low molecular weight protein. (M.tb)

96

Belongs to theESAT-6 (esx) family. Molecular function protein binding. Involved in virulence. Immunogenic. [18, 24, 58–61]