Differential cellular recognition pattern to M. tuberculosis targets defined by IFN-γ and IL-17 production in blood from TB + patients from Honduras as compared to health care workers: TB and immune responses in patients from Honduras

Nancy Alvarez-Corrales; Raija K Ahmed; Carol A Rodriguez; Kithiganahalli N Balaji; Rebeca Rivera; Ramakrishna Sompallae; Nalini K Vudattu; Sven E Hoffner; Alimuddin Zumla; Lelany Pineda-Garcia; Markus Maeurer

doi:10.1186/1471-2334-13-125

BMC Infectious Diseases

Table 1 Summary of M.tb test targets

From: Differential cellular recognition pattern to M. tuberculosis targets defined by IFN-γ and IL-17 production in blood from TB + patients from Honduras as compared to health care workers: TB and immune responses in patients from Honduras

Peptides
Gene locus	RefSeq	M.tbAntigens	aa	Comment
Rv0447c	NP854118 (Pool 1)	Probable cyclopropane fatty acyl phospholipid synthase.	427	Methyl transferase activity. Cyclopropane fatty acyl phospholipid synthase activity. Lipid biosynthetic process [17, 18]
Rv2940c	YP_976584 (Pool 2)	Mycocerosic acid synthase	2111	Lipid biosynthetic process. Oxido-reduction and transferase activity, Cofactor binding. Location at the cell wall. [17, 19–23]
Rv3347c	YP_177963 (Pool 3)	PPE family protein	3157	Function unknown. Gly-Ala-Asn rich protein, interacts with the host system by inhibition of antigen processing. [18, 23–26]
Rv2453c	CAA16030 (Pool 4)	Probable molybdopterin-guanine dinucleotide biosynthesis Protein A	201	Molybdenum cofactor biosynthesis. Molecular function as GTP binding. Located at the cytoplasm membrane. [18, 23, 24, 27]
Rv1886c	CAB10044 (Pool 5)	Antigen 85B	325	Fibronectin binding protein. Acyltransferase activity. Secreted protein, also located at cell wall, plasma membrane. [18, 28–31]
Rv1690	CAB10947 (Pool 6)	Probable lipoprotein	127	Putative uncharacterized protein. Protein binding, cellular component plasma membrane.[18, 24, 32]
Rv3019c	CAA16104 (Pool 7)	ESAT-6 like protein	96	Belongs to ESAT-6 (esx) family, Protein-protein interaction [18, 24]
Rv2957	CAB05419 (Pool 8)	PGL/p-HBAD biosynthesis glycosyltrans-ferase MT3031	256	Glycosyl transferase activity, transferring hexosyl groups. Glycolipid biosynthetic function. Identified as a drug target. [18, 24, 30, 33, 34]
Rv1085c	CAA17201 (Pool 9)	UPF0073 membrane protein	242	Belongs to the UPF0073 (HIy-III) family. Cytolysis. Sub cellular location in cell membrane [18, 24]
Rv0066c	CAA16247 (Pool 10)	Isocitrate dehydrogenase, NADP-dependent- icd2.	745	Oxidoreductase function. NAD or NADH binding. Isocitrate dehidrogenase (NADP+) activity. Magnesium ion binding. Protein homodimerization. [18, 22–24, 35, 36]
Rv2958c	CAB05418 (Pool 11)	PGL/p-HBAD biosynthesis glycosyltrans-ferase	428	Glycolipid biosynthetic process, pathogenesis, Glycosyl transferase activity. Immune evasion or - tolerance [18, 22, 24, 33, 37]
Rv2962c	CAB05415 (Pool 12)	PGL/p-HBAD biosynthesis rhamnosyl-transferase	449	Glycolipid biosynthetic process, pathogenesis. Glycosyl transferase activity. Evasion or tolerance concerning to the host immune response. [18, 22, 24, 30, 33, 37]
Rv3804c/1886	CAA17868/CAB10044	Ag85A/Ag85B Fibronecting binding protein peptide pool	338/325	Belongs to the Ag85 family, contains Esterase D. Possesses mycolyl transferase activity. [28, 29, 38–41]
Proteins
Rv3804c	CAA17868	Ag85A. Secreted antigen 85A. Mycolyl transferase 85A. fbpA. Ag85 complex	338	Responsible for high affinity of mycobacteria to fibronectin. Possesses mycolyl transferase for biogenesis of trehalose dimycolate. [22, 23, 29, 30, 38–40, 42–44]
Rv1886c	CAB10044	Ag85B. Secreted antigen 85B. Mycolyl transferase 85B. fbpB. Ag85 complex	325	Fibronectin binding protein. Acyltransferase activity. Response to antibiotic. Secreted protein also located at cell wall, extracellular region. [28–30, 36, 38, 40, 41, 45]
Rv3875	CAA56099	ESAT-6. 6 KDa early secretory antigenic target. esxA	95	Function unknown. Elicit high level of IFNgamma during the first phase of protective immune response. Secreted protein, cell wall and cytoplasm. [46–54]
Rv3874	CAA17966	CFP-10. 10 KDa culture filtrate antigen	100	ESAT-6 like protein esxB. Forms a tight 1:1 complex with EsxA. Protein binding. Host cell surface binding. Pathogenesis. Protein secretion. [22, 47–49, 51, 53, 54]
Rv0754	CAE55319	PE_PGR11. PE-PGRS family protein	584	Member of M.tb PE family. PGRS subfamily gly-rich proteins. Unknown function. Protein existence predicted. [18, 21]
Rv0978c	CAE55343	PE_PGR17. PE-PGRS family protein	331	Member of M.tb PE family. PGRS subfamily gly-rich proteins. Unknown function. Protein existence predicted. [18, 55, 56]
Rv1917c	CAE55440	PPE34. PPE family protein	1459	Member of M.tb PPE family. Glycin rich proteins. Unknown function. [18, 57]
Rv0288	CAA17363	TB10.4. Low molecular weight protein. (M.tb)	96	Belongs to theESAT-6 (esx) family. Molecular function protein binding. Involved in virulence. Immunogenic. [18, 24, 58–61]

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