Table 1 Parameters during each round of the SELEX assay
SELEX rounds | Antibody (μg/well) | ssDNA(μg/well) | Reaction time in BSA-blocked blank wells (min) | Reaction time in antibody-coated wells (min) |
|---|---|---|---|---|
1 | 1 | 0.5 | 15 | 60 |
2 | 0.5 | 0.5 | 20 | 60 |
3 | 0.25 | 0.5 | 20 | 60 |
4 | 0.25 | 0.1 | 30 | 45 |
5 | 0.1 | 0.1 | 30 | 45 |
6 | 0.25 | 0.1 | 30 | 45 |
7 | 0.1 | 0.1 | 45 | 30 |
8 | 0.1 | 0.05 | 45 | 30 |
9 | 0.05 | 0.05 | 45 | 30 |
10 | 0.05 | 0.025 | 60 | 20 |
11 | 0.025 | 0.025 | 60 | 20 |
12 | 0.025 | 0.01 | 60 | 15 |