Frequency of Chlamydia trachomatis in Ureaplasma-positive healthy women attending their first prenatal visit in a community hospital in Sapporo, Japan

Table 1 Primer sequences and PCR conditions used for this study

Microorganism	Target gene	Primer	Sequence	Annealing condition;	Expected	Reference [Reference number]
				Temperature/cycle	fragment size
Most bacteria	16S rRNA	Forward (Bac3)	5'-AGA GTT TGA TYM TGG CTC AG-3'*	52/35	nearly full-length	Horn et al., 1999 [22]
		Reverse (Bac4)	5'-CAK AAA GGA GGT CC-3'**
Chlamydia trachomatis	ompA	Forward (CtOmp1)	5'-ATG AAA AAA CTC TTG AAA TCG G-3'	55/35	1,100 bp	Jurstrand et al., 2010 [23]
		Reverse (CtOmp2)	5'-ACT GTA ACT GCG TAT TTG TCT G-3'
Ureaplasma parvum	MBA gene with	Forward (UMS-57)	5'-(T/C)AA ATC TTA GTG TTC ATA TTT TTT AC-3'	58/35	326/327 bp	Kong et al., 2000 [13]
	it upstream region***	Reverse (UMA222)	5'-GTA AGT GCA GCA TTA AAT TCA ATG-3'
Ureaplasma urealyticum	MBA gene with it upstream region	Forward (UMS-170)	5'-GTA TTT GCA ATC TTT ATA TGT TTT CG-3'	58/45	476 bp	Kong et al., 2000 [13]
		Reverse (UMA263)	5'-TTT GTT GTT GCG TTT TCT-3'

ISSN: 1471-2334