Table 1 Primers and probes used to detect TSPyV and MCPyV
Virus | Primers & probes sequence (5′-->3′) | Region | Amplicon size |
|---|---|---|---|
MCPyV | FWD-CCACAGCCAGAGCTCTTCCT | LT | 140 |
REV-TGGTGGTCTCCTCTCTGCTACTG | |||
FAM-TCCTTCTCAGCGTCCCAGGCTTCA-TAMRA | |||
MCPyV | FWD-TGCCTCCCACATCTGCAAT | VP1 | 59 |
REV-GTGTCTCTGCCAATGCTAAATGA | |||
6FAM-TGTCACAGGTAATATC-MGBNFQ | |||
TSPyV | FWD-TGTGTTTGGAAACCAGAATCATTTG | LT | 140 |
REV-TGCTACCTTGCTATTAAATGTGGAG | |||
FAM-TTCTTCTTCCTCCTCATCCTCCACCTCAAT-BHQ1 | |||
TSPyV | FWD-AGTCTAAGGACAACTATGGTTACAG | VP1 | 140 |
REV-ATTACAGGTTAGGTCCTCATTCAAC | |||
FAM-ACAGCAGTGACCAGGACAAGCCTACTTCTG-BHQ1 | |||
Tail Sequence | AACTGACTAAACTAGGTGCCACGTCGTGAAAGTCTGACAAGTGTCTCTGCCAA TGCTAAATGA | ||