Analysis of activated T-cell subpopulations after culture. Flow cytometry was performed to assess the expression of the HLA-DR+ activation marker on CD4+, CD8+ and CD28+ cells in peripheral blood. Lymphocyte phenotyping was performed to assess the activation of CD4+ and CD8+ cells after 120 hours of culture. PBMCs were stimulated with soluble egg antigen (SEA) and soluble worm antigen preparation (SWAP). Specific gating strategies to select the T cells subsets are represented by the dot plots (J and K). The results are expressed as the median (interquartile range) for each group BD: n = 11, NI: n = 13 and XTO: n = 10. The egg counts in the XTO group ranged from 12 to 96 eggs/g of stool (mean = 54 eggs/g). The Kruskal-Wallis and Dunn's test post-test were performed for statistical analysis.