Figure 4

Enhanced DEN-2 replication but suppressed IL12B in heterotypic antibody-mediated DEN-2 infected differentiated THP-1 cells. (A) To compare viral replication, viral load of DEN-2 in antiserum-mediated DEN-2 infection was determined by TaqMan real-time RT-PCR (DEN-2 (■), anti-DEN1 antiserum + DEN-2 (░), and anti-DEN2 antiserum + DEN-2 (□), p = 0.022, 0.042, and 0.777 respectively) after infection of DEN-2 for 24 h in a summary calculated from the results of 5-paired experiments. Suppression of IL12B levels in differentiated THP-1 cells was found in the addition of anti-DEN1 antiserum diluted to 1:1250 after infection of DEN-2 for 24 h (B) (DEN-2 (■), anti-DEN1 antiserum + DEN-2 (░), and anti-DEN2 antiserum + DEN-2 (□), p = 0.024, 0.014, and 0.054 respectively) after infection of DEN-2 for 24 h in a summary calculated from the results of 5-paired experiments.