Figure 3

Dose-dependent and kinetic induction of IFNA and IL12B expression in DEN-2-infected THP-1 cells with or without PMA differentiation. A series of M.O.I. of DEN-2 were assessed at post-infection 24 h, RNA samples from THP-1 (A) or PMA-differentiated THP-1 (C) cells were subjected to testing IFNA and IL12B mRNAs by SYBR Green real-time RT-PCR analysis. A different post-infection time of DEN-2 were assessed at M.O.I.=1, RNA samples from THP-1 (B) or PMA-differentiated THP-1 (D) cells were subjected to testing IFNA and IL12B mRNAs by SYBR Green real-time RT-PCR analysis. The similar dose response (E) and kinetic induction (F) of IL-12 p40 proteins in the supernatants of DEN-2 infected PMA-differentiated THP-1 cells by ELISA in the same reactions.