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Table 2 Evaluation of r-Δgp160-based TRF assays using worldwide HIV performance panel (WWRB 302)

From: Escherichia coli–expressed near full length HIV-1 envelope glycoprotein is a highly sensitive and specific diagnostic antigen

Member ID#

Origin

Gtpa

Abbott

Gen. Sys.

OT HIV1b

TRF assaysc

HIV1b

HIV1/2b

HIV1b

HIV1/2b

r-Δgp160 two-step

r-Δgp160 one-step

01

Spain

O

1.1

1.8

0.8

5.6

1.3

1.3 (+)

0.6 (−)

02

Ghana

A

>11.5

>16.1

6.9

8.7

7.0

94.0 (+)

9.2 (+)

03

Ghana

G

>11.5

>16.1

7.1

8.8

7.2

164.8 (+)

41.6 (+)

04

Ghana

G

>11.5

>16.1

7.1

8.8

6.5

138.0 (+)

4.1 (+)

05

Ghana

A

>11.5

>16.1

7.1

8.7

7.0

253.6 (+)

82.2 (+)

06

Ghana

G

>11.5

>16.1

6.9

8.8

7.1

91.4 (+)

15.1 (+)

08

Ivory Coast

G

>11.5

>16.1

6.9

8.7

6.7

73.0 (+)

4.4 (+)

09

Ivory Coast

A

>11.5

>16.1

6.9

8.6

6.5

107.8 (+)

3.9 (+)

10

Ivory Coast

Neg

0.4

0.2

0.1

0.4

0.4

0.7 (−)

0.4 (−)

11

Mozambique

HIV-2

1.2

14.6

0.6

9.7

3.0

1.0 (+)

0.7 (−)

12

Mozambique

C

>11.5

>16.1

7.1

8.9

6.9

187.1 (+)

4.1 (+)

14

Uganda

D

>11.5

>16.1

4.5

8.5

6.2

53.3 (+)

13.8 (+)

15

Uganda

D

>11.5

>16.1

6.3

8.1

7.2

132.9 (+)

16.8 (+)

16

Uganda

D

>11.5

>16.1

7.0

8.8

6.9

154.5 (+)

7.3 (+)

17

Uganda

D

>11.5

>16.1

6.8

9.8

7.0

131.2 (+)

5.1 (+)

19

Zimbabwe

C

>11.5

>16.1

6.0

9.9

7.0

41.4 (+)

10.3 (+)

21

China

B

>11.5

>16.1

6.7

8.8

7.0

136.7 (+)

26.8 (+)

22

Thailand

E

>11.5

>16.1

7.3

9.8

7.0

100.8 (+)

7.0 (+)

24

Thailand

E

>11.5

>16.1

7.4

9.8

6.9

88.4 (+)

23.2 (+)

25

India

HIV-2

0.4

15.4

3.8

10

2.1

4.9 (+)

1.5 (+)

26

USA

D

>11.5

>16.1

7.4

9.8

7.1

85.8 (+)

48.5 (+)

27

USA

B/D

>11.5

>16.1

7.0

9.8

7.2

135.7 (+)

12.7 (+)

28

Argentina

F

>11.5

>16.1

7.0

8.9

6.8

72.5 (+)

3.0 (+)

29

Argentina

B

>11.5

>16.1

6.9

8.5

6.6

92.9 (+)

2.2 (+)

30

Argentina

Neg

0.3

0.2

0.2

0.2

0.4

0.9 (−)

0.4 (−)

  1. aGenotype.
  2. bValues indicate S/Co ratios, provided by the panel supplier using the indicated commercial EIA kits. S/Co values ≥ 1.0 are considered as positive. (Gen. Sys., Genetic Systems; OT, Organon Teknika).
  3. cValues indicate S/Co ratios obtained using r-Δgp160-based TRF assays. The results are indicated in parentheses. Samples with S/Co values < 1.0 are designated as negative (−) and those with values ≥ 1.0 are designated as positive (+).