Figure 2

SDS-PAGE analysis of r-Δgp160 and r-Trx-BAP-Δgp160 antigens. Aliquots of total lysates of E. coli harboring the r-Δgp160 and r-Trx-BAP-Δgp160 antigen constructs, before (lanes ‘U’) and after (lanes ‘I’) IPTG induction, and aliquots of the affinity-purified antigens (lanes ‘P’), were electrophoresed on denaturing gels and visualized by Coomassie staining. Protein size markers were run in lane ‘M’; their sizes (in kDa) are shown on the left. The arrows shown on the right denote r-Trx-BAP-Δgp160 (upper) and r-Δgp160 (lower), respectively. The asterisk denotes the position of biotin ligase enzyme.