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Table 3 Comparison of the frequently identified bacterial genera detected by molecular and culture testing

From: Clinical identification of bacteria in human chronic wound infections: culturing vs. 16S ribosomal DNA sequencing

16S DNA Sequencing Culture
Samples Genus Samples Genus
46 Staphylococcus* 28 Staphylococcus*
38 Corynebacterium* 21 Enterococcus*
32 Pseudomonas* 8 Pseudomonas*
31 Clostridium 8 Serratia*
28 Bacillus* 7 Proteus
27 Enterococcus* 6 Corynebacterium*
26 Paenibacillus 4 Enterobacter*
22 Serratia* 2 Citrobacter*
22 Propionibacterium 2 Escherichia*
20 Escherichia* 2 Streptococcus*
20 Streptococcus* 1 Acinetobacter*
19 Brevibacterium 1 Aeromonas
17 Citrobacter* 1 Bacillus*
17 Klebsiella* 1 Klebsiella*
17 Lactobacillus   
17 Finegoldia   
15 Sarcina   
13 Anaerococcus   
11 Bacteroides   
11 Peptoniphilus   
11 Prevotella   
10 Enterobacter*   
10 Salmonella   
9 Providencia   
9 Peptostreptococcus   
8 Acinetobacter*   
8 Nocardiopsis   
  1. Of the 145 genera identified by 16S sequencing in the 51 samples in this study, the 27 most frequently identified genera are included in the table. Nine (9) of these 27 genera are obligate anaerobes, and culture did not attempt to detect these organisms. All 14 of the bacterial genera identified by culture are included in the table. The two bacteria detected by culture and not listed in the “16S DNA Sequencing” column of the table are Proteus and Aeromonas, which were detected using 16S sequencing 6 and 1 times, respectively. The frequency of identification of each genus is noted under the “Samples” heading.
  2. * Genus present in both columns.
  3. Obligate anaerobic genus.