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Table 3 Comparison of the frequently identified bacterial genera detected by molecular and culture testing

From: Clinical identification of bacteria in human chronic wound infections: culturing vs. 16S ribosomal DNA sequencing

16S DNA Sequencing

Culture

Samples

Genus

Samples

Genus

46

Staphylococcus*

28

Staphylococcus*

38

Corynebacterium*

21

Enterococcus*

32

Pseudomonas*

8

Pseudomonas*

31

Clostridium

8

Serratia*

28

Bacillus*

7

Proteus

27

Enterococcus*

6

Corynebacterium*

26

Paenibacillus

4

Enterobacter*

22

Serratia*

2

Citrobacter*

22

Propionibacterium

2

Escherichia*

20

Escherichia*

2

Streptococcus*

20

Streptococcus*

1

Acinetobacter*

19

Brevibacterium

1

Aeromonas

17

Citrobacter*

1

Bacillus*

17

Klebsiella*

1

Klebsiella*

17

Lactobacillus

  

17

Finegoldia

  

15

Sarcina

  

13

Anaerococcus

  

11

Bacteroides

  

11

Peptoniphilus

  

11

Prevotella

  

10

Enterobacter*

  

10

Salmonella

  

9

Providencia

  

9

Peptostreptococcus

  

8

Acinetobacter*

  

8

Nocardiopsis

  
  1. Of the 145 genera identified by 16S sequencing in the 51 samples in this study, the 27 most frequently identified genera are included in the table. Nine (9) of these 27 genera are obligate anaerobes, and culture did not attempt to detect these organisms. All 14 of the bacterial genera identified by culture are included in the table. The two bacteria detected by culture and not listed in the “16S DNA Sequencing” column of the table are Proteus and Aeromonas, which were detected using 16S sequencing 6 and 1 times, respectively. The frequency of identification of each genus is noted under the “Samples” heading.
  2. * Genus present in both columns.
  3. Obligate anaerobic genus.