Clinical identification of bacteria in human chronic wound infections: culturing vs. 16S ribosomal DNA sequencing

BMC Infectious Diseases

Table 1 Comparison of the number of bacterial taxa detected using molecular testing and culturing testing

	Mean	STD	Min	Max
16S Testing for All Bacterial Genera
All Genera	14.8	7.5	3	38
Dominant Genera	2.2	1.1	1	5
Major Genera	3.7	3.7	0	16
Minor Genera	8.9	5.7	0	26
16S Testing for Aerotolerant Bacterial Genera
All Genera	9.8	5.3	2	29
Dominant Genera	1.8	0.9	0	4
Major Genera	2.4	2.3	0	10
Minor Genera	5.6	4.4	0	19
Bacterial Genera Detected by Aerobic Culture
All Genera	1.8	0.9	1	5

The number of bacterial genera identified using molecular testing and the number of bacterial genera cultured using aerobic testing of the 51 samples are listed. The mean number of genera identified using molecular identification or isolates identified using culture identification are listed. Molecular testing results are listed for both 1) all bacterial genera and for 2) only aerotolerant bacterial genera. The standard deviation (STD), minimum number of genera (Min), and the maximum number of genera (Max) are also listed. These same data were determined for subsets of genera determined to be present at >10% of the relative abundance in the sample (Dominant), 1-10% of the relative abundance in the sample (Major), and less than 1% of the relative abundance in the sample (Minor).

ISSN: 1471-2334