The percentage of viral reduction in Vero cells after different treatments with RC-1. (A) RC-1 was diluted to various concentrations (37.5, 75, 100, 125, 150 and 175 μM) with DMEM media supplemented with 2 % FBS and added to the cell culture. The viability of cells was analyzed at 24, 48 and 72 hrs using Non-Radioactive Cell Proliferation assay. (B) The cells were grown under the optimal conditions and infected with DENV-2 at the MOI of 2. The MNTD of RC-1 (150 μM) was used to treat infected Vero cells and the results were normalized with infected cells without RC-1 as controls. Pre, simultaneous and post-infection treatments with RC-1 were applied at 24, 48 and 72 hrs of each infection. The percentage of inhibition was the highest for simultaneous treatment compared to pre and post-treatment. (Two way ANOVA was used with Bonferroni post-test, P < 0.0001).