Figure 2

Antimicrobial activity of recombinant RC-1 after refolding. Each sample of RC-1 before and after refolding (0.1 mg/ml) was dropped on filter papers discs and left to dry at room temperature. The dry filter paper discs were placed on E. coli and yeast-inoculated antibiotic-free Mueller-Hinton-gar and YPD-agar plates respectively and incubated overnight at 37°C (E. coli) or 30°C (yeast). Recombinant RC-1 exhibited antimicrobial activity against E. coli (A) and yeast (Saccharomyces cerevisiae) (B) compared to mis-folded form and control plates (column buffer) as exhibited by the presence of a clear circular zone in the agar plate.