Table 1 Primer pairs used to quantify CHIKV, DENV, and WNV by qRT-PCR
From: Aedes albopictus in Lebanon, a potential risk of arboviruses outbreak
Virus | Viral gene | Sense primer (5’-3’) | Anti-sense primer (5’-3’) | Fragment size (bp) | Reference |
|---|---|---|---|---|---|
CHIKV | E2 | 9018 – CAC CGC CGC AAC TAC CG | 9235 - GAT TGG TGA CCG CGG CA | 217 | [25] |
DENV-2 | C | 153 - GAG AAA CCG CGT GTC AAC TG | 266- GGA AAC GAA GGA ATG CCA CC). | 113 | modified from [32] |
WNV | C/M | 175 - GTG TTG GCT CTC TTG GCG TT | 279 - AGG TGT TTC ATC GCT GTT TG | 104 | [33] |