Number of viral particles (A) and transmission rate (B) of
SARBA at day 10 post-infection. We exposed mosquitoes to an infectious blood-meal containing CHIKV, DENV-2 or WNV at a titer of 108 MID50/ml. At day 10 pi, wings and legs of 20 mosquitoes were removed and the proboscis inserted into a capillary tube filled with L15 medium supplemented with 10% FBS. After 45 min, saliva was collected and titrated by fluorescent foci method on C6/36 cells. Transmission rate corresponds to the number of infected saliva among tested ones. In brackets, the number of mosquitoes analyzed.