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Table 1 Limited virucidal activity of ethanol, isopropanol and peracetic acid against duck hepatitis B virus (DHBV) depending on concentration and contact time in comparison to vaccinia virus (VACV)/modified vaccinia Ankara strain (MVA)

From: Does limited virucidal activity of biocides include duck hepatitis B virucidal action?

Biocide Concentration (%) Contact time (min) Reduction factors
DHBV VACV/MVA
Ethanol 30 1 2.99 ± 0.78 −0.10 ± 0.29/0.01 ± 0.26
  2 3.46 ± 0.64 0.32 ± 0.28/-0.01 ± 0.30
40 1 4.35 3.28/2.94 ± 0.27
  2 4.38 4.59/≥4.05
50 1 4.84 5.11/≥4.76
  2 4.88 5.11/≥5.76
60 1 4.88 5.11/≥5.76
   2 4.88 5.11/≥5.76
Isopropanol 20 1 1.96 ± 0.66 0.01 ± 0.29/0.08 ± 0.25
  2 2.33 ± 0.61 −0.01 ± 0.30/-0.04 ± 0.30
30 1 3.39 ± 0.88 1.63 ± 0.30/1.35 ± 0.22
  2 4.10 ± 0.80 1.87 ± 0.26/2.67 ± 0.27
40 1 4.00 5.11 1/≥4.76 1
   2 4.00 5.11 1/≥4.76 1
Peracetic acid 0.001 1 1.36 ± 0.35 0.74 ± 0.31/0.74 ± 0.27
  2 1.67 ± 0.41 1.62 ± 0.30/1.79 ± 0.30
0.005 1 2.43 ± 0.51 4.50/≥5.66
  2 3.05 ± 0.39 4.50/≥5.66
0.01 1 3.55 ± 0.60 4.48/≥4.85
  2 4.08 4.48/≥4.85
0.05 1 4.08 4.15/≥4.35
   2 4.08 4.15/≥4.35
  1. Results for VACV and MVA are given as means calculated from the results of three different laboratories (Rabenau et al. [10]).
  2. “≥” - Determination of RF is limited by hepatocytotoxic reactions and/or the amount. Calculation of 95% confidence interval is not possible.
  3. 1 Results are only from one laboratory.