SNP-based real-time pyrosequencing as a sensitive and specific tool for identification and differentiation of Rickettsia species in Ixodes ricinus ticks

Table 2 Overlapping primers for Rickettsia positive control plasmids

	Forward primer	Reverse primer
R. helvetica	5’-CGTGCCGCAGTACTTAAAGAAACTTGTAAGGAAGTA TTAAAGGAACTCGGACAGCTAGAAAACAATCCGC-3’	5’-CATCTTTAAGAGCGATAGCTTCAAGTTCTATTGCT ATTTGTAAGAGCGGATTGTTTTCTAGCTGTCCGAG-3’
R. monacensis	5’-CGTGCCGCAGTACTTAAAGAAACGTGCAAAGAAGT ATTAAAGGAACTCGAACAGTTAGAAAATAATCCAC-3’	5’-CATCTTTAAGAGCGATAGCTTCAAGTTCTATTGCT ATTTGTAAAAGTGGATTATTTTCTAACTGTTCGAG-3’
R. massiliae	5’-CGTGCCGCAGTACTTAAAGAAACGTGCAAAGAAGT ATTAAAGGAACTCGGGCAGTTAGACAACAATCCGC-3’	5’-CATCTTTAAGAGCGATAGCTTCAAGTTCTATTGCT ATTTGTAAGAGCGGATTGTTGTCTAACTGCCCGAG-3’
R. felis 1	5’-CGTGCCGCAGTACTTAAAGAAACCTGCAAAGAAGT ATTAAAGGAACTTGGACAGCTAGAAAACAACCCAC-3’	5’-CATCTTTAAGAGCGATAGCTTCAAGTTCTATTGCT ATTTGCAAGAGTGGGTTGTTTTCTAGCTGTCCAAG-3’
R. felis 2	5’-CGTGCCGCAGTACTTAAAGAAACTTGCAAAGAAGT ATTAAAGGAACTCGGACAGCTAGAAAACAATCCGC-3’	5’-CATCTTTAAGAGCGATAGCTTCAAGTTCTATTGCT ATTTGCAAAAGCGGATTGTTTTCTAGCTGTCCGAG-3’

ISSN: 1471-2334