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Table 2 Overlapping primers for Rickettsia positive control plasmids

From: SNP-based real-time pyrosequencing as a sensitive and specific tool for identification and differentiation of Rickettsia species in Ixodes ricinus ticks

  Forward primer Reverse primer
R. helvetica 5’-CGTGCCGCAGTACTTAAAGAAACTTGTAAGGAAGTA TTAAAGGAACTCGGACAGCTAGAAAACAATCCGC-3’ 5’-CATCTTTAAGAGCGATAGCTTCAAGTTCTATTGCT ATTTGTAAGAGCGGATTGTTTTCTAGCTGTCCGAG-3’
R. monacensis 5’-CGTGCCGCAGTACTTAAAGAAACGTGCAAAGAAGT ATTAAAGGAACTCGAACAGTTAGAAAATAATCCAC-3’ 5’-CATCTTTAAGAGCGATAGCTTCAAGTTCTATTGCT ATTTGTAAAAGTGGATTATTTTCTAACTGTTCGAG-3’
R. massiliae 5’-CGTGCCGCAGTACTTAAAGAAACGTGCAAAGAAGT ATTAAAGGAACTCGGGCAGTTAGACAACAATCCGC-3’ 5’-CATCTTTAAGAGCGATAGCTTCAAGTTCTATTGCT ATTTGTAAGAGCGGATTGTTGTCTAACTGCCCGAG-3’
R. felis 1 5’-CGTGCCGCAGTACTTAAAGAAACCTGCAAAGAAGT ATTAAAGGAACTTGGACAGCTAGAAAACAACCCAC-3’ 5’-CATCTTTAAGAGCGATAGCTTCAAGTTCTATTGCT ATTTGCAAGAGTGGGTTGTTTTCTAGCTGTCCAAG-3’
R. felis 2 5’-CGTGCCGCAGTACTTAAAGAAACTTGCAAAGAAGT ATTAAAGGAACTCGGACAGCTAGAAAACAATCCGC-3’ 5’-CATCTTTAAGAGCGATAGCTTCAAGTTCTATTGCT ATTTGCAAAAGCGGATTGTTTTCTAGCTGTCCGAG-3’