Table 1 Comparison of Sanger sequencing and real-time pyrosequencing
Sensitivity | |||
|---|---|---|---|
Copy number in initial template (2 μl) | Sanger Sequencing | real-time pyrosequencing | P-value |
| Â | Number of identified tick samples (%) | Â | |
<101 | 1/73 (1.4) | 11/73 (15.1) | 0.013* |
101-102 | 5/24 (8.3) | 13/24 (54.2) | 0.180 |
>102-103 | 28/45 (62.2) | 43/45 (95.6) | 0.239 |
>103-104 | 72/74 (97.3) | 74/74 (100) | 0.999 |
>105-105 | 42/42 (100) | 42/42 (100) | 1.000 |
>105-106 | 4/4 (100) | 4/4 (100) | 1.000 |
>106 | 1/1 (100) | 1/1 (100) | 1.000 |
all samples | 153/263 (58.2) | 188/263 (71.5) | 0.002* |