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Table 1 Comparison of Sanger sequencing and real-time pyrosequencing

From: SNP-based real-time pyrosequencing as a sensitive and specific tool for identification and differentiation of Rickettsia species in Ixodes ricinus ticks

Sensitivity
Copy number in initial template (2 μl) Sanger Sequencing real-time pyrosequencing P-value
  Number of identified tick samples (%)  
<101 1/73 (1.4) 11/73 (15.1) 0.013*
101-102 5/24 (8.3) 13/24 (54.2) 0.180
>102-103 28/45 (62.2) 43/45 (95.6) 0.239
>103-104 72/74 (97.3) 74/74 (100) 0.999
>105-105 42/42 (100) 42/42 (100) 1.000
>105-106 4/4 (100) 4/4 (100) 1.000
>106 1/1 (100) 1/1 (100) 1.000
all samples 153/263 (58.2) 188/263 (71.5) 0.002*
  1. * Statistically significant.