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Table 4 PCR/Electron spray ionization-time-of-flight-mass spectrometry (PCR/ESI-TOF-MS) versus traditional clinical microbiology (TCM) for detection of most frequently recovered potentially clinically relevant organisms, plus coagulase negative staphylococci (CNS), contaminating high-use surfaces

From: Comparison of PCR/Electron spray Ionization-Time-of-Flight-Mass Spectrometry versus Traditional Clinical Microbiology for active surveillance of organisms contaminating high-use surfaces in a burn intensive care unit, an orthopedic ward and healthcare workers

Bacteria PCR/ESI-TOF-MS (# isolates) n = 305 TCM (# isolates) n = 89
  Bedrail Door handle Faucet IV pump Keyboard Mouse Shears Bedrail Door handle Faucet IV pump Keyboard Mouse Shears
CNS 24 31 28 23 30 22 19 11 6 14 9 16 9 7
Enterococci*   2      1 2 1   1    
S. aureus 4   1 4 2 3   3   1 1 1 2  
Streptococci** 7 8 12 12 17 7 5        1
Acinetobacter spp.*** 2 1 2 6      1   1    
Enterobacter spp.**** 3 1 1 1     1 1      
E. coli 2 1   1           
K. pneumoniae 7   1 3 1 3         
Pseudomonas spp.***** 4   2   1          
Candida spp.****** 7 3 5 3 1 2         
  1. *TCM: “Enterococcus spp.”; PCR/ESI-TOF-MS: 2 Enterococcus faecalis, 1 Enterococcus faecium.
  2. **TCM: 1 viridans group streptococci; PCR/ESI-TOF-MS: 1 Group D Streptococcus, 24 S. agalactiae, 8 Streptococcus pneumoniae, 1 S. pyogenes, 1 “Streptococcus spp.”, 33 viridans group streptococci.
  3. ***TCM: 2 A. baumannii; PCR/ESI-TOF-MS: 10 A. baumannii, 1 “Acinetobacter spp.”
  4. ****TCM: 2 Enterobacter cloacae; PCR/ESI-TOF-MS: 4 E. cloacae; 2 Enterobacter sakazakii.
  5. *****PCR/ESI-TOF-MS: 1 P. aeruginosa, 2 Pseudomonas entomophila/putida, 3 P. fluorescens, 1 “Pseudomonas spp.”
  6. ******PCR/ESI-TOF-MS: 10 C. albicans, 4 C. glabrata, 1 Candida kefyr, 1 Candida kruseii, 1 Candida parapsilosis, 1 Candida rugosa, 3 Candida tropicalis.
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BMC Infectious Diseases

ISSN: 1471-2334

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