Skip to main content

Table 4 PCR/Electron spray ionization-time-of-flight-mass spectrometry (PCR/ESI-TOF-MS) versus traditional clinical microbiology (TCM) for detection of most frequently recovered potentially clinically relevant organisms, plus coagulase negative staphylococci (CNS), contaminating high-use surfaces

From: Comparison of PCR/Electron spray Ionization-Time-of-Flight-Mass Spectrometry versus Traditional Clinical Microbiology for active surveillance of organisms contaminating high-use surfaces in a burn intensive care unit, an orthopedic ward and healthcare workers

Bacteria

PCR/ESI-TOF-MS (# isolates) n = 305

TCM (# isolates) n = 89

 

Bedrail

Door handle

Faucet

IV pump

Keyboard

Mouse

Shears

Bedrail

Door handle

Faucet

IV pump

Keyboard

Mouse

Shears

CNS

24

31

28

23

30

22

19

11

6

14

9

16

9

7

Enterococci*

 

2

    

1

2

1

 

1

   

S. aureus

4

 

1

4

2

3

 

3

 

1

1

1

2

 

Streptococci**

7

8

12

12

17

7

5

      

1

Acinetobacter spp.***

2

1

2

6

    

1

 

1

   

Enterobacter spp.****

3

1

1

1

   

1

1

     

E. coli

2

1

 

1

          

K. pneumoniae

7

 

1

3

1

3

        

Pseudomonas spp.*****

4

 

2

 

1

         

Candida spp.******

7

3

5

3

1

2

        
  1. *TCM: “Enterococcus spp.”; PCR/ESI-TOF-MS: 2 Enterococcus faecalis, 1 Enterococcus faecium.
  2. **TCM: 1 viridans group streptococci; PCR/ESI-TOF-MS: 1 Group D Streptococcus, 24 S. agalactiae, 8 Streptococcus pneumoniae, 1 S. pyogenes, 1 “Streptococcus spp.”, 33 viridans group streptococci.
  3. ***TCM: 2 A. baumannii; PCR/ESI-TOF-MS: 10 A. baumannii, 1 “Acinetobacter spp.”
  4. ****TCM: 2 Enterobacter cloacae; PCR/ESI-TOF-MS: 4 E. cloacae; 2 Enterobacter sakazakii.
  5. *****PCR/ESI-TOF-MS: 1 P. aeruginosa, 2 Pseudomonas entomophila/putida, 3 P. fluorescens, 1 “Pseudomonas spp.”
  6. ******PCR/ESI-TOF-MS: 10 C. albicans, 4 C. glabrata, 1 Candida kefyr, 1 Candida kruseii, 1 Candida parapsilosis, 1 Candida rugosa, 3 Candida tropicalis.