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Table 3 PCR/Electron spray ionization-time-of-flight-mass spectrometry (PCR/ESI-TOF-MS) versus traditional clinical microbiology (TCM) for detection of most frequently recovered potentially clinically relevant pathogens, plus coagulase negative staphylococci (CNS), contaminating healthcare workers and personal protective equipment

From: Comparison of PCR/Electron spray Ionization-Time-of-Flight-Mass Spectrometry versus Traditional Clinical Microbiology for active surveillance of organisms contaminating high-use surfaces in a burn intensive care unit, an orthopedic ward and healthcare workers

  PCR/ESI-TOF-MS (n = 77) TCM (n = 23)
Organism Hands pre-care Gloves Gowns Hands post-care Hands pre-care Gloves Gowns Hands post -care
CNS 12 6 8 12 4 4 3 3
Staphylococcus aureus   3 3 1   3 1 1
Streptococci* 9 4 3 1   1   
Acinetobacter spp.**   3 1    1   
Enterobacter spp.***   1     2   
Escherichia coli   1 1      
Klebsiella pneumoniae 1 3 1 1     
Pseudomonas spp.**** 1    1     
Candida spp.*****   2       
  1. *TCM: 1 Group D Streptococcus; PCR/ESI-TOF-MS: 7 S. agalactiae, 10 viridans group streptococci.
  2. **TCM: 1 A. baumannii; PCR/EIS-MS: 2 A. baumannii, 2 A. calcoaceticus.
  3. ***TCM: 2 Enterobacter aerogenes; PCR/ESI-TOF-MS: 1 E. aerogenes.
  4. ****PCR/ESI-TOF-MS: 1 Pseudomonas fluorescens, 1 Pseudomonas mendocina.
  5. *****PCR/ESI-TOF-MS: 1 C. albicans, 1 Candida glabrata.