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Table 1 Primer sequences and probes used in real-time PCR assay

From: Excessive proinflammatory cytokine and chemokine responses of human monocyte-derived macrophages to enterovirus 71 infection

Gene

Primer and probe sequence (5′to3′)

Product length(bp)

GenBank

EV71

(R) CCCGCTCTGCTGAAGAAACT

89

AF302996

 

(F)AGTGATGAGAGTATGATTGAGACACG

  
 

(P) TCGCACAGCACAGCTGAGACCACTC

  

β-actin

(R) CAAGTACTCCGTGTGGATCG

90

NM_001101.3

 

(F) GGATGCAGAAGGAGATCACTG

  
 

(P) CCCTGGCACCCAGCACAATGA

  

RIG-1

(R) CCTCTGCACTGTTGCTCAGGAC

192

NM_004585.3

 

(F) CTCTTGGCTTCGAGATGGCTTC

  

MDA5

(R) ATTGGTGACGAGACCATAACGGATA

196

NM_022168.2

 

(F) AGGAGTCAAAGCCCACCATCTG

  

TLR2

(R) CACAAAGTATGTGGCATTGTCCAG

158

NM_003264.3

 

(F) GTGTTGCAAGCAGGATCCAAAG

  

TLR3

(R) AGTGCCGTCTATTTGCCACACA

181

NM_003265.2

 

(F) AACAGTGCACTTGGTGGTGGAG

  

TLR4

(R) ATGCGGACACACACACTTTCAAATA

143

NM_138554.3

 

(F) TTGAGCAGGTCTAGGGTGATTGAAC

  

TLR6

(R) AGCCTTCAGCTTGTGGTACTTGTTG

138

NM_006068.2

 

(F) CAGAGTGAGTGGTGCCATTACGA

  

TLR7

(R) TCTTCAACCAGACCTCTACATTCCA

172

NM_016562.3

 

(F) GGAACATCCAGAGTGACATCACAG

  

TLR8

(R) GATTGCTGCACTCTGCAATAACTGA

196

NM_138636.3

 

(F) GCTGCTGCAAGTTACGGAATGA

  

TLR9

(R) CAGGGCCTTCAGCTGGTTTC

97

NM_017442.2

 

(F) CCGTGACAATTACCTGGCCTTC

  

TLR10

(R) TGTGTTGCAAGATAATTCGTGGAGA

103

NM_001017388.1

 

(F) CATGATGGTTGGATGGTCAGATTC

  

β-actin

(R) CTAAGTCATAGTCCGCCTAGAAGCA

186

NM_001101.3

 

(F) TGGCACCCAGCACAATGAA