burdens in various tissues of guinea pigs with abraded skin inoculation. Visualization of leptospires (brown particles and threads) in the dermis and subcutaneous (a), muscular layers (b and c), liver (d), hemorrhagic area of lungs (e), none hemorrhagic area of lungs (f), kidney (g) and retroperitoneum (h) of guinea pigs with leptospires inoculation on abraded skin. Black arrow indicates leptospires in the cytoplasm of phagocytes. Tissues were observed at 2 h p.i. (a), 24 h p.i. (b and c) and 144 h p.i. (d-h). Leptospires were stained by immunohistochemistry. (EnVision, magnification, × 400).