Expression of CD27 and CD28 in Flu-MP* peptide-specific CD8
T cells. (A) Flow cytometry dot plot of CD27 and CD28 expression in one representative chagasic patient. (B) There were no differences between the early differentiation phenotype (CD27+ CD28+) and the intermediate/late differentiation stage (CD27+ CD28-, CD27- CD28-) in CD8+ T cells (P > 0.151). There were no significant differences between the chagasic patients (white bars) and healthy donors (grey bars) when comparing the early phenotype (P = 0.139) with intermediate/late differentiation stage (P = 0.430).