Figure 2

PCR-based diagnosis and Leishmania species assignation. PCR products from 12 samples from patients using V1-V2 primers for Viannia subgenus identification. Lanes 1, 4, 5, 8, 9 and 13 were positives, and lanes 2, 3, 10, 11, 12 and 14 were negatives. Lanes 6 and 7 are the negative and positive control, respectively (A). PCR using M1-M2 primers for L. mexicana and L. donovani complexes applied to five DNA samples. Lanes 1 to 5 were negatives. Lanes 6 and 7 negative and positive control, respectively (B). Species identification by PCR analysis with primers b1-b2 (b), g1-g2 (g) and p1-p2 (p) specific for L. (V.) braziliensis, guyanensis and panamensis, respectively (C). Patient 1: Lane b = positive, g and p = negatives. Patient 2: g = positive, b and p = negatives. Patient 3: p = positive, b and g = negatives. Lanes 4 and 5, negative and positive control, respectively. M: marker of molecular weight. Arrows indicate the expected location of the bands.