The development of a GeXP-based multiplex reverse transcription-PCR assay for simultaneous detection of sixteen human respiratory virus types/subtypes

Table 2 Detection of 16 respiratory viruses in 126 specimens

Virus	No. of specimens				Performance of the GeXP assay
	GeXP + ^aRVP + ^a	GeXP + RVP -	GeXP- RVP +	GeXP-^aRVP -^a	Sensitivity %	Specificity %	PPV^a%	NPV^a%
FluA	5	0	0	121	100	100	100	100
sH1N1^b	3	0	0	123	100	100	100	100
FluB	1	0	0	125	100	100	100	100
PIV1	1	0	0	125	100	100	100	100
PIV2	1	0	0	125	100	100	100	100
PIV3	21	3^d	0	102	100	97.14	87.50	100
HRV^c	68	6	3	49	95.77	89.09	91.89	94.23
HMPV	8	0	2	116	80.00	100	100	98.31
Adv	9	0	1	116	90.00	100	100	99.15
CoV NL63	1	0	0	125	100	100	100	100
CoV OC43	12	0	0	114	100	100	100	100
CoV 229E	2	0	0	124	100	100	100	100
CoV HKU1	4	0	0	122	100	100	100	100
RSVA^b	2	0	0	124	100	100	100	100
RSVB^b	46	0	3	77	93.88	100	100	96.25
HBoV	15	0	4	107	78.95	100	100	96.40

^a The numbers of positive and negative specimens detected by the GeXP assay are indicated as GeXP + and GeXP -, respectively, and the numbers of positive and negative specimens detected by the RVP Fast assay are indicated as RVP + and RVP -, respectively. The sensitivity (True positives, TP), specificity (True negatives, TN), PPV (TP/TP + false positives), and NPV (TN/TN + false negatives) for each target are calculated using the RVP Fast assay as the reference for comparison.
^b The RVP Fast assay was able to subtype the FluA and RSV positive specimens.
^c The RVP Fast assay was not able to distinguish rhinovirus from enterovirus, so ‘HRV’ positive detected by the RVP Fast assay could be HRV or enterovirus. All of the specimens positive for HRV detected by the GeXP assay were confirmed by sequencing as true positives.
^d All of the additional PIV3 detected only by the GeXP assay were confirmed by sequencing as true positives.

ISSN: 1471-2334