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Table 2 Detection of 16 respiratory viruses in 126 specimens

From: The development of a GeXP-based multiplex reverse transcription-PCR assay for simultaneous detection of sixteen human respiratory virus types/subtypes

Virus No. of specimens Performance of the GeXP assay
  GeXP + aRVP + a GeXP + RVP - GeXP- RVP + GeXP-aRVP -a Sensitivity % Specificity % PPVa% NPVa%
FluA 5 0 0 121 100 100 100 100
sH1N1b 3 0 0 123 100 100 100 100
FluB 1 0 0 125 100 100 100 100
PIV1 1 0 0 125 100 100 100 100
PIV2 1 0 0 125 100 100 100 100
PIV3 21 3d 0 102 100 97.14 87.50 100
HRVc 68 6 3 49 95.77 89.09 91.89 94.23
HMPV 8 0 2 116 80.00 100 100 98.31
Adv 9 0 1 116 90.00 100 100 99.15
CoV NL63 1 0 0 125 100 100 100 100
CoV OC43 12 0 0 114 100 100 100 100
CoV 229E 2 0 0 124 100 100 100 100
CoV HKU1 4 0 0 122 100 100 100 100
RSVAb 2 0 0 124 100 100 100 100
RSVBb 46 0 3 77 93.88 100 100 96.25
HBoV 15 0 4 107 78.95 100 100 96.40
  1. a The numbers of positive and negative specimens detected by the GeXP assay are indicated as GeXP + and GeXP -, respectively, and the numbers of positive and negative specimens detected by the RVP Fast assay are indicated as RVP + and RVP -, respectively. The sensitivity (True positives, TP), specificity (True negatives, TN), PPV (TP/TP + false positives), and NPV (TN/TN + false negatives) for each target are calculated using the RVP Fast assay as the reference for comparison.
  2. b The RVP Fast assay was able to subtype the FluA and RSV positive specimens.
  3. c The RVP Fast assay was not able to distinguish rhinovirus from enterovirus, so ‘HRV’ positive detected by the RVP Fast assay could be HRV or enterovirus. All of the specimens positive for HRV detected by the GeXP assay were confirmed by sequencing as true positives.
  4. d All of the additional PIV3 detected only by the GeXP assay were confirmed by sequencing as true positives.