Specificity and sensitivity analyses of the GeXP Assay. The Y-axis indicates the dye signal in arbitrary units, and the X-axis indicates the actual PCR products size. Panels A-P show the results of amplification of FluA (H3N2), FluB, FluA (sH1N1), PIV1, PIV2, PIV3, HRV, HMPV, Adv, RSVA, RSVB, HBoV, CoV OC43, CoV 229E, CoV NL63 and CoV HKU1, respectively. Nuclease-free water was used as a negative control (NC). All of the 16 pre-mixed viral targets could be detected at the level of 1000 copies of each virus per reaction in the multiplex assay (R). The viral targets, from left to right, are HRV, RSVA, FluB, CoV NL63, CoV 229E, PIV2, CoV OC43, HMPV, CoV HKU1, PIV3, sH1N1, FluA, RSVB, PIV1, HBoV and Adv (R).