Detection of hydrogen peroxide-producing Lactobacillus species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women

Table 2 Prevalence of Lactobacillus species detected using broad range PCR among a subset of culture samples*

Species	Number of samples with species detected¹n = 16
L. gasseri	13 (81)
L. crispatus	2 (13)
L. iners ²	2 (13)
L. johnsonii	2 (13)
L. coleohominis	1 (6)
L. oris	1 (6)
L. reuteri	1 (6)
L. rhamnosus	1 (6)

*Samples from women with high quantities of H₂O₂-producing Lactobacillus by culture (≥ 10⁶ CFUs), but were negative for both L. crispatus and L. jensenii in CVL by qPCR.
¹Percent total >100% since several isolates contained more than one species.
²H₂O₂-functionality was reassessed for the 2 isolates that were identified as L. iners. No H₂O₂-production was observed.

ISSN: 1471-2334