Table 3 Cytokine levels in serum and in cell supernatants before and after doxycycline and placebo treatment
Cytokine | Antigen | Doxycycline median pg/ml (Interquartile range) | Placebo median pg/ml (Interquartile range) | Detectable (%) | ||
|---|---|---|---|---|---|---|
Before | After | Before | After | |||
Serum | ||||||
IL-1β | 0.43 (0.13-1.50) | 0.60 (0.13-1.10) | 0.52 (0.13-0.70) | 0.60 (0.13-0.84) | 68 | |
IL-6 | 0.19 (0.19-1.60) | 0.19 (0.19-2.70) | 0.19 (0.19-2.70) | 0.19 (0.19-3.20) | 43 | |
IL-8 | 8.15 (6.0-12.9) | 9.78 (7.70-14.3) | 10.6 (7.40-14.9) | 10.1 (7.90-13.7) | 100 | |
GM-CSF | 0.75 (0.75-9.85) | 0.75 (0.75-9.10) | 0.75 (0.75-10.2) | 0.75 (0.75-11.5) | 40 | |
IL-10 | 0.15 (0.15-1.80) | 0.15 (0.15-1.72) | 0.15 (0.15-1.30) | 0.15 (0.15-1.97) | 45 | |
Supernatants | ||||||
IL-1β | OF | 267 (147–416) | 276 (178–353) | 258 (160–372) | 257 (212–325) | 100 |
LPS | 373 (297–578) | 336 (273–459) | 384 (306–464) | 376 (272–417) | 100 | |
IL-6 | OF | 8495 (5577–11552) | 10018 (7283–11436) | 9017 (7893–12936) | 10549 (6765–11870) | 97 |
LPS | 7457 (5522–9344) | 7043 (6173–9401) | 7339 (5640–9010) | 7129 (6164–8376) | 100 | |
IL-8 | OF | 234726 (182996–353519) | 256238 (224077–323425) | 264450 (187637–377221) | 254926 (343734) | 100 |
LPS | 170005 (84896–191690) | 158091 (116470–210940) | 159121 (119796–175857) | 153546 (203419) | 100 | |
unstim. | 949 (425–3337) | 887 (412–2657) | 966 (580–2637) | 728 (443–1324) | 100 | |
TNF | OF | 49.4 (41.8-72.4) | 51.8 (26.9-210) | 91.2 (66.7-141) | 69.3 (25.8-152) | 77 |
LPS | 45.0 (23.8-61.3) | 45.4 (31.7-56.4) | 36.7 (27.1-57.5) | 37.1 (26.4-51.9) | 77 | |
IL-10 | OF | 50.6 (41.8-84.7) | 59.3 (48.4-84.7) | 64.4 (46.3-100) | 67.3 (47.0-86.3) | 82 |
LPS | 177 (130–272) | 184 (114–259) | 201 (154–255) | 181 (119–280) | 98 | |