PCR results targeting the
16S-23S rRNA intergenic spacer region using primers 325-1000 and primers 425-1100 (top row) and
specific primers (bottom row). With primers 325-1000, amplicons were obtained in lanes 1-3 from the resected liver lobe (pre-antibiotic treatment) but not in Lanes 4-5 (post-treatment liver biopsy). With primers 425-1000, single amplicons were obtained in the pre- (Lane 2) and post-treatment (Lane 5) liver samples. By DNA sequencing, all amplicons corresponded to a 16S-23S strain of B. henselae. No amplicons were obtained with the B. koehlerae primer set. RC = DNA extraction control, lane, BH = B. henselae (Houston I strain), PO = PCR negative control, M = Kb DNA ladder.