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Table 3 Comparison of RVP, RespiFinder-19, RespiFinder-SMART-22 and monoplex real-time PCR with regard to the analytical sensitivity in a sample inoculated with 4 viruses

From: Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay

 

RVP

RespiFinder-19

RespiFinder-SMART-22

Monoplex real-time PCR (CT)

 

und.

1:10

1:100

und.

1:10

1:100

und.

1:10

1:100

und.

1:10

1:100

Panel 1

55 μl elution

55 μl elution

55 μl elution

55 μl elution

(INF-A H1/ RSV-A/ HRV/ AdV)

+ / + / + / +

+ / + / + / -

+ / - / + / -

- / + / + / +

- / - / + / -

- / - / - / -

- / + / + / +

- / - / + / -

- / - / + / -

21.8/22.3/ 20.4/28.7

25.8/26.9/ 26.8/30.5

29.6/28.3/ 30.8/31.7

 

100 μl elution

100 μl elution

100 μl elution

 
 

+ / + / + / +

+ / + / + / -

+ / - / + / -

+ / - / + / +

+ / - / + / -

- / - / - / -

- / - / + / +

- / - / + / +

- / - / + / +

   

Panel 2*

55 μl elution

55 μl elution

55 μl elution

55 μl elution

(INF-A H1/ INF-A H3/ RSV-A/ PIV-2)

+ / + / + / +

+ / + / + / -

+ / + / - / -

- / - / + / +

- / - / + / +

- / - / + / +

- / - / + / +

- / - / + / +

- / - / + / +

24.3/24.3/ 22.8/20.4

28.7/28.7/ 25.5/26.4

31.1/31.1/ 28.7/30.4

 

100 μl elution

100 μl elution

100 μl elution

 
 

+ / + / + / +

+ / + / + / -

+ / + / - / -

+ / + / - / +

+ / + / - / +

+ / + / - / -

+ / + / + / +

+ / + / + / +

+ / + / - / +

   

Panel 3*

55 μl elution

55 μl elution

55 μl elution

55 μl elution

(CoV OC43/ CoV 229E/ AdV/ HRV)

+ / - / + / +

+ / - / + / +

- / - / - / +

+ / + / + / +

+ / + / + / +

+ / + / + / -

+ / + / + / +

+ / + / + / +

+ / + / + / +

25.0/22.3/ 29.7/22.4

28.7/25.3/ 31.8/25.4

32.0/26.5/ 32.6/28.5

 

100 μl elution

100 μl elution

100 μl elution

 
 

+ / - / + / +

+ / - / + / +

- / - / - / +

+ / + / + / +

+ / + / + / +

+ / + / + / -

+ / + / + / +

+ / + / + / +

+ / + / + / +

   
  1. *Concentration of viruses are concordant to Table 1.