Table 3 Comparison of RVP, RespiFinder-19, RespiFinder-SMART-22 and monoplex real-time PCR with regard to the analytical sensitivity in a sample inoculated with 4 viruses
RVP | RespiFinder-19 | RespiFinder-SMART-22 | Monoplex real-time PCR (CT) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
und. | 1:10 | 1:100 | und. | 1:10 | 1:100 | und. | 1:10 | 1:100 | und. | 1:10 | 1:100 | |
Panel 1 | 55 μl elution | 55 μl elution | 55 μl elution | 55 μl elution | ||||||||
(INF-A H1/ RSV-A/ HRV/ AdV) | + / + / + / + | + / + / + / - | + / - / + / - | - / + / + / + | - / - / + / - | - / - / - / - | - / + / + / + | - / - / + / - | - / - / + / - | 21.8/22.3/ 20.4/28.7 | 25.8/26.9/ 26.8/30.5 | 29.6/28.3/ 30.8/31.7 |
100 μl elution | 100 μl elution | 100 μl elution | ||||||||||
+ / + / + / + | + / + / + / - | + / - / + / - | + / - / + / + | + / - / + / - | - / - / - / - | - / - / + / + | - / - / + / + | - / - / + / + | ||||
Panel 2* | 55 μl elution | 55 μl elution | 55 μl elution | 55 μl elution | ||||||||
(INF-A H1/ INF-A H3/ RSV-A/ PIV-2) | + / + / + / + | + / + / + / - | + / + / - / - | - / - / + / + | - / - / + / + | - / - / + / + | - / - / + / + | - / - / + / + | - / - / + / + | 24.3/24.3/ 22.8/20.4 | 28.7/28.7/ 25.5/26.4 | 31.1/31.1/ 28.7/30.4 |
100 μl elution | 100 μl elution | 100 μl elution | ||||||||||
+ / + / + / + | + / + / + / - | + / + / - / - | + / + / - / + | + / + / - / + | + / + / - / - | + / + / + / + | + / + / + / + | + / + / - / + | ||||
Panel 3* | 55 μl elution | 55 μl elution | 55 μl elution | 55 μl elution | ||||||||
(CoV OC43/ CoV 229E/ AdV/ HRV) | + / - / + / + | + / - / + / + | - / - / - / + | + / + / + / + | + / + / + / + | + / + / + / - | + / + / + / + | + / + / + / + | + / + / + / + | 25.0/22.3/ 29.7/22.4 | 28.7/25.3/ 31.8/25.4 | 32.0/26.5/ 32.6/28.5 |
100 μl elution | 100 μl elution | 100 μl elution | ||||||||||
+ / - / + / + | + / - / + / + | - / - / - / + | + / + / + / + | + / + / + / + | + / + / + / - | + / + / + / + | + / + / + / + | + / + / + / + | ||||