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Table 3 Comparison of RVP, RespiFinder-19, RespiFinder-SMART-22 and monoplex real-time PCR with regard to the analytical sensitivity in a sample inoculated with 4 viruses

From: Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay

  RVP RespiFinder-19 RespiFinder-SMART-22 Monoplex real-time PCR (CT)
  und. 1:10 1:100 und. 1:10 1:100 und. 1:10 1:100 und. 1:10 1:100
Panel 1 55 μl elution 55 μl elution 55 μl elution 55 μl elution
(INF-A H1/ RSV-A/ HRV/ AdV) + / + / + / + + / + / + / - + / - / + / - - / + / + / + - / - / + / - - / - / - / - - / + / + / + - / - / + / - - / - / + / - 21.8/22.3/ 20.4/28.7 25.8/26.9/ 26.8/30.5 29.6/28.3/ 30.8/31.7
  100 μl elution 100 μl elution 100 μl elution  
  + / + / + / + + / + / + / - + / - / + / - + / - / + / + + / - / + / - - / - / - / - - / - / + / + - / - / + / + - / - / + / +    
Panel 2* 55 μl elution 55 μl elution 55 μl elution 55 μl elution
(INF-A H1/ INF-A H3/ RSV-A/ PIV-2) + / + / + / + + / + / + / - + / + / - / - - / - / + / + - / - / + / + - / - / + / + - / - / + / + - / - / + / + - / - / + / + 24.3/24.3/ 22.8/20.4 28.7/28.7/ 25.5/26.4 31.1/31.1/ 28.7/30.4
  100 μl elution 100 μl elution 100 μl elution  
  + / + / + / + + / + / + / - + / + / - / - + / + / - / + + / + / - / + + / + / - / - + / + / + / + + / + / + / + + / + / - / +    
Panel 3* 55 μl elution 55 μl elution 55 μl elution 55 μl elution
(CoV OC43/ CoV 229E/ AdV/ HRV) + / - / + / + + / - / + / + - / - / - / + + / + / + / + + / + / + / + + / + / + / - + / + / + / + + / + / + / + + / + / + / + 25.0/22.3/ 29.7/22.4 28.7/25.3/ 31.8/25.4 32.0/26.5/ 32.6/28.5
  100 μl elution 100 μl elution 100 μl elution  
  + / - / + / + + / - / + / + - / - / - / + + / + / + / + + / + / + / + + / + / + / - + / + / + / + + / + / + / + + / + / + / +    
  1. *Concentration of viruses are concordant to Table 1.