Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay

Table 2 Comparison of RVP, RespiFinder-19, RespiFinder-SMART-22 and monoplex real-time PCR with regard to the analytical sensitivity in a sample with 13 viruses

Virus	Concentration [copies/ml]	RVP				RespiFinder-19				RespiFinder- SMART-22				Monoplex real-time PCR
		und.	1:10	1:100	1:1000	und.	1:10	1:100	1:1000	und.	1:10	1:100	1:1000	und.	1:10	1:100	1:1000
INF-A H1	1.78E+04	+	+	+	-	-	-	-	-	-	-	-	-	32.9	33.8	35.8	37.9
INF-A H3	1.42E+04	+	+	+	-	-	-	-	-	-	-	-	-
INF-B	n. q.	-	-	-	-	-	-	-	-	-	-	-	-	x	x	x	x
RSV-A	3.94E+04	+	+	+	-	-	-	-	-	-	-	-	-	28.7	30.4	32.5	34.6
RSV-B	n. q.	+	+	+	-	-	-	-	-	-	-	-	-	x	x	x	x
PIV-1	1.61E+04	-	-	-	-	-	-	-	-	-	-	-	-	32.8	33.1	34.9	36.6
PIV-2	9.19E+03	-	-	-	-	+	-	-	-	+	+	+	-	29.5	31.4	33.7	36.4
PIV-3	3.84E+04	-	-	-	-	-	-	-	-	-	-	-	-	34.9	36.8	38.3	39.8
CoV OC43	n. q.	+	+	+	+	+	+	+	+	+	+	+	+	27.6	28.9	30.1	31.9
CoV 229E	n. q.	-	-	-	-	+	+	+	+	+	+	+	+	26.8	27.9	29.1	30.5
HRV	2.83E+04	+	+	+	+	+	+	-	-	+	+	+	-	31.2	33.8	36.0	38.4
AdV	3.56E+04	-	-	-	-	+	+	+	+	+	+	+	-	35.9	37.6	38.8	39.9
hMPV	1.78E+04	+	+	+	+	-	-	-	-	-	-	-	-	30.2	32.0	34.8	38.4

n. q.: not quantified; x: not performed; und.: undiluted; The dilution series were done as two-fold dilution series.

ISSN: 1471-2334