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Table 2 Comparison of RVP, RespiFinder-19, RespiFinder-SMART-22 and monoplex real-time PCR with regard to the analytical sensitivity in a sample with 13 viruses

From: Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay

Virus Concentration [copies/ml] RVP RespiFinder-19 RespiFinder- SMART-22 Monoplex real-time PCR
   und. 1:10 1:100 1:1000 und. 1:10 1:100 1:1000 und. 1:10 1:100 1:1000 und. 1:10 1:100 1:1000
INF-A H1 1.78E+04 + + + - - - - - - - - - 32.9 33.8 35.8 37.9
INF-A H3 1.42E+04 + + + - - - - - - - - -     
INF-B n. q. - - - - - - - - - - - - x x x x
RSV-A 3.94E+04 + + + - - - - - - - - - 28.7 30.4 32.5 34.6
RSV-B n. q. + + + - - - - - - - - - x x x x
PIV-1 1.61E+04 - - - - - - - - - - - - 32.8 33.1 34.9 36.6
PIV-2 9.19E+03 - - - - + - - - + + + - 29.5 31.4 33.7 36.4
PIV-3 3.84E+04 - - - - - - - - - - - - 34.9 36.8 38.3 39.8
CoV OC43 n. q. + + + + + + + + + + + + 27.6 28.9 30.1 31.9
CoV 229E n. q. - - - - + + + + + + + + 26.8 27.9 29.1 30.5
HRV 2.83E+04 + + + + + + - - + + + - 31.2 33.8 36.0 38.4
AdV 3.56E+04 - - - - + + + + + + + - 35.9 37.6 38.8 39.9
hMPV 1.78E+04 + + + + - - - - - - - - 30.2 32.0 34.8 38.4
  1. n. q.: not quantified; x: not performed; und.: undiluted; The dilution series were done as two-fold dilution series.