The influence of prednisolone on CD4 counts, CD4/CD8 ratio and naïve T cells. CD4 counts and CD4/CD8 ratio were determined from blood samples by routine diagnostic. Whole blood from donors of different treatment groups was stained with fluorescent dye-coupled anti-CD3, anti-CD4, anti-CD62L and anti-CD45RA antibodies. Cells were analyzed by flow cytometry after erythrocyte lysis. Lymphocytes were gated in a FSC/SSC scatter. CD3+/CD4+/CD62L+/CD45RA cells from the lymphocyte gate were scored as naïve CD4+ T cells, CD3+/CD4-/CD62L+/CD45RA cells were scored as naïve CD8+ T cells. Horizontal bars represent medians. Statistical analysis was performed using a Mann-Whitney test. A: absolute CD4+ T cell counts. B: CD4/CD8 T cell ratio. C: percentage of naïve CD8+ T lymphocytes. D: percentage of naïve CD4+ T lymphocytes.