Evidence for an enterovirus as the cause of encephalitis lethargica

BMC Infectious Diseases

Table 1 Immunohistochemical analysis using anti-poliovirus polyclonal antibody

Neutralizing dose/ml	0.42	0.2	0.1	0.05	0 Antibody
Controls, UK
06/112	2+	2+	1+
06/110	2+	2+	0		0
06/126/7	0
05/152-9	2+	3+	0.5+
05/25-	1+				0
NP86/07	0
05/47-11	0.5
05/137/5	0
05/66-28	0
05/01-	0.5
Controls, USA
865183	0
8889	1+
8854127	0.5+
883599	3+		2+		0
1311056	1+
Guam 644255	0.5			0
EL, USA
21225	2+	4+	2+	2+	0
19940	3+	3+	3+	2+	0
91558		4+	2+	1+	1+
28065	3+	1+	2+	0	0

Immunohistochemical analysis using anti-poliovirus types 1, 2 and 3 rabbit polyclonal antibody. Each type was diluted according to neutralizing titer to create equivalence of titer for the three polio types. The antibody was pooled and absorbed with human brain tissue (see Materials). Antibody quantitation is expressed as units of neutralizing dose (N. D.). Grades of staining intensity are expressed as follows: 0 – 0.5+ = no staining; 1+ = background intensity and not significant; 2+ − 4+ = significant staining of increasing intensity. Fractions of grades are the mean of more than one observation at the same experiment.

ISSN: 1471-2334