Figure 3

Effects of low dose ATL 370 and alanyl-glutamine on C. difficile toxin A-induced secretion and epithelial injury. Varying doses of alanyl-glutamine (AQ; mM) in the lumen when given in addition to intravenous ATL 370 (1.5 ng) showed decrease in toxin A (10 ug)-induced intraluminal fluid accumulation (A) and epithelial injury (B) in rabbit ileal loops. Panel 3A: a- P = 0.08 (one-way ANOVA), and b (PBS vs. Toxin A)- P = 0.0002, c (Toxin A vs ATL/AQ10)- P = 0.02, d (Toxin A vs ATL/AQ30)- P = 0.01, e (Toxin A vs ATL/AQ100)- P = 0.0004 (Unpaired t-test with Welch's). Panel 3B: a- P < 0.0001 (one-way ANOVA), and b(PBS vs. Toxin A)- P < 0.001, c (Toxin A vs ATL/AQ10)- P < 0.01, d (Toxin A vs ATL/AQ30)- P < 0.01, e (Toxin A vs ATL/AQ100)- P < 0.001 (Bonferroni's). Results were data pooled from 4 to 16 loops from 2 rabbits per group from 2 separate experiments.