Level of proinflammatory cytokines (TNF-α, IFN-γ, MCP-1, IL-6, IL-10 and IL-12p70) in sera of C3H/HeJ and C3H/CaJ mice. At various time points, three mice from both infected (inf) as well as control (con) mice were bled. The sera were separated and used for cytokine quantification. The levels of TNF-α, MCP-1, IL-10, IL-6, IL-12 and IFN-γ in the undiluted sera were quantified using a BD™ Cytometric Bead Assay mouse inflammation kit. The beads were acquired and analyzed using a FACSCalibur cell sorter and BD™ Cytometric Bead Array Software (BD Bioscience). The level of cytokines was quantified using known standard supplied with kit. Values represent the mean ± SE of three mice.