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Figure 4 | BMC Infectious Diseases

Figure 4

From: Blood leukocytes from benznidazole-treated indeterminate chagas disease patients display an overall type-1-modulated cytokine profile upon short-term in vitro stimulation with trypanosoma cruzi antigens

Figure 4

Analysis of intracytoplasmic cytokine profile of B-cells in the peripheral blood from untreated. Indeterminate chagasic patients (IND gray rectangle), Bz-treated Indeterminate chagasic patients (INDt black rectangle) as compared to non-infected individual (NI empty rectangle). Data are expressed as median absolute counts plus the interquartile range of cytokine+ cells observed after short-term in vitro “Control Culture” (left panel) and T. cruzi epimastigote antigen “Stimulated Culture” (right panel) of whole-blood samples. Cytokine+ B-cells were quantified by dual color immunophenotyping (anti-CD19-TC plus anti-cytokine-PE mAbs) using specific gate strategy, as described in Material and Methods. The significant differences at p < 0.05 for comparisons with NI are indicated by the letter ‘a’. The significant differences at p < 0.05 for comparisons with INDt are indicated by connecting lines. The significant differences at p < 0.05 for comparative analysis between “Control” and “Stimulated” cultures are indicated by *.

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