Identification of tuberculosis-associated proteins in whole blood supernatant

BMC Infectious Diseases

Table 2 Characteristics of proteins identified in this study.

A: Comparison between patients with active TB and control subjects
		2D-DIGE			LC-MS/MS
Condition	Serial Number	PDQ SSP#^a	P value	+/-^b	Swiss-Plot	Mascot search score^e	Protein name	Da	pI
Patients versus control subjects	1	HT6102	0.0064	-	RET4_HUMAN	72	Retinol binding protein 4	23010	5.76
	2	HT2406	0.0097	-	FETUA_HUMAN	75	α-2-HS-glycoprotein	39325	5.43
	3	HT5401	0.0331	+	VTDB_HUMAN	98	Vitamin D binding protein	52964	5.40
	4	HT2303	0.0419	+	CO4A_HUMAN	86	Complement C4A	192771	6.66
	5	HT1012	0.0271	-	APOC3_HUMAN	105	Apolipoprotein C-III	10852	5.23
	6	HT5303	< 0.001	-	APOA4_HUMAN	190	Apolipoprotein A-IV	45399	5.28
	7	HT1016	0.0024	-	APOC2_HUMAN	61	Apolipoprotein C-II	11284	4.72
B: Comparison between stimulated and unstimulated conditions in active TB
		2D-DIGE			LC-MS/MS
Condition	Serial Number	PDQ SSP#	P value	+/- ^c	Swiss-Plot	Mascot search score	Protein name	Da	pI
Mitogen (versus no stimuli)	8	T3601	0.0917	-	C1S_HUMAN	169	Complement-C1S	76684	4.86
	9	T3403	0.0156	+	KNG1_HUMAN	139	Kininogen-1	71957	6.34
	10	T3105	0.0866	-	ZA2G_HUMAN	45	Zinc-α-2-glycoprotein	33872	5.57
	11	T4512	0.0061	-	A1BG_HUMAN	76	α-1B-glycoprotein	54273	5.58
Mtb antigens (versus no stimuli)	12	T4203	0.0640^d	+	CLUS_HUMAN	47	Clusterin	52495	5.89
	13	T3107	0.0687^d	+	CLUS_HUMAN	50	Clusterin	52495	5.89
	14	T4208	0.0732^d	+	EST	--	Clusterin	52495	5.89

^aPDQ SSP# is a PDQuest software standard spot number indicating the unique location of each spot automatically assigned on a 2D gel and is essential for comparing the same spots on different gels.
^bThe average density of a spot from 2D-DIGE is higher (+) or lower (-) in patients with active TB than in control subjects.
^cThe average density of the spot from 2D-DIGE is higher (+) or lower (-)under the stimulated condition than that under the unstimulated condition.
^dThe average density of the 3 spots, T4203, T3107, and T4208, which correspond to a subset of clusterin, was significantly higher in Mtb-specific antigen-stimulated than in unstimulated samples (P = 0.0014).
^eThe Mascot search score indicates the degree of compatibility between mass spectra generated by the sample and amino acid sequences within the protein of interest.

ISSN: 1471-2334